Want to know more about
the technology of bioinformatics?
Consult this recent textbook from the Cambridge University Press, an
introduction to Unix and Perl for life scientists without previous
knowledge of such tools.
Welcome to Webcutter 2.0! This new version of Webcutter is a complete rewrite. Along with cleaner and more maintainable code, I am pleased to introduce the following new features:
Rainbow cutters Highlight your favorite enzymes in color or boldface for easy at-a-glance identification
Silent cutters Find sites which may be introduced by silent mutagenesis of your coding sequence
Sequence uploads Input sequences directly into Webcutter from a file on your hard drive without needing to cut-and-paste
Degenerate sequences Analyze restriction maps of sequences containing ambiguous nucleotides like N, Y, and R.
Circular sequences Choose whether to treat your sequence as linear or circular
Enzyme info Click into the wealth of references and ordering information at New England BioLabs' REBASE, directly from your restriction map results
Plus all of the features Webcutter has always had, from automatic sequence search-and-entry from NCBI's GenBank to its easy customizable interface and clean simple results format. For a mini-manual on how Webcutter 2.0 works, how to get the most from it, and some of its known limitations, please click here.
There are three ways to input your sequence:
You can copy-and-paste it or type it into the box below
You can upload a sequence file from your computer by clicking the "Browse..." button below. If you are not using Netscape, you may not see the browse button and cannot use this feature (sorry).
Or, you can search NCBI's GenBank for your sequence by entering search terms below. Webcutter will submit your search to GenBank and automatically retrieve and enter your sequence for you.
For more advanced searches, please visit one of these sites